09/22/2004
One Egg - Many Siblings
On September 11, my wife and I drove to the Brandywine region of Pennsylvania to visit my brother and his wife, who have moved to a retirement community close to Longwood Gardens. Earlier that day, I had watched some of the 9/11 anniversary ceremonies in New York in which parents and grandparents were reciting the names of the victims of the World Trade Center attack. For those of us in the New York area, the wound of 9/11 is still raw and there was criticism that in at least one section of New York there was a festive street fair in progress that day.
Therefore, it felt strange that evening to find ourselves sitting on a lawn in Pennsylvania watching fireworks, definitely not the way New Yorkers would mark the occasion. The fireworks, in Longwood Gardens, were the most impressive and unusual fireworks I’ve ever seen, with overlapping rings, multicolored rings and rings with puffs of light above and below them. Not being in the Gardens, we weren’t privy to any patriotic words or music that accompanied the fireworks, but assume that they were in good taste.
During our visit, my brother and I reminisced about our youth and I was amazed to find that we shared one particular memory. In 1941, our mother learned to drive so she could assist our father on the long drive from our home in Mechanicsburg, Pennsylvania to visit our grandparents in Denver, Colorado. There were no interstate highways in those days. My mother was passing a truck on a road outside Red Oak, Iowa and found herself partly on the left-hand shoulder. She seemed not to have the car under complete control and Dad grabbed the wheel. We swung across the road and slammed into a guardrail that prevented us from going down an embankment. Happily, the truck driver had slowed down and didn’t hit us.
Our hood flew up and the horn was blowing. My brother, 10 at the time, said to our mother, “I knew it. I knew you’d do it!” We now agree that our father shared responsibility for the accident. We ended up in a garage in Red Oak, left the car there to be fixed and caught a train to Denver. I can still see Grandma and Grandpa greeting us in the Denver station. It’s no surprise that both my brother and I remember the accident quite clearly. What surprised me was that, 63 years later, my brother said that the water in that Red Oak garage was the best water he’s ever tasted. I too can still taste that water. Were we both in shock and revived by the water or was it really outstanding water? We’ll never know.
During our visit, I also found there was sibling rivalry that I, being the older brother, had not recognized. I had preceded my brother at Dickinson College by several years. When he arrived there, he suffered the inevitable comparisons with his brother and resolved to follow a different path. I went to Pitt for my graduate work; he went to Penn State, Pitt’s archrival at the time. I worked at Bell Labs; he was a professor at the University of Delaware. Neither of us realized, however, that our paths were remarkably similar. We both initially had visions of becoming organic chemists. Strangely, the professor at Dickinson who turned me on to organic chemistry turned my brother off the subject. I was turned off by a course in organic chemistry at Pitt taught by Professor Dull. He was a very nice man but the course was indeed dull! My brother and I both ended up being physical chemists.
One day, after returning from our visit, I found an angry wasp trapped between a window and the screen and spent about a half hour getting it into a position where I could dispatch him (or her). I was musing about the wasp and my discovery of the sibling rivalry when I ran across an article by Andy Gardner and Stuart West in the September 3 issue of Science. The article is titled “Spite Among Siblings” and deals with sibling interactions in, appropriately, wasps! The wasp in question is Copidosoma sosares, a “polyembryonic” parasitic wasp. Polyembryony was new to me and even the explanatory statement “A single wasp egg proliferates asexually (clonally) to produce multiple larvae…” left me unsatisfied.
Visits to the Web sites of the Natural Environment Research Council in the UK and the honorary fraternity Sigma Xi enlightened me. In a polyembryonic parasitic wasp, the female wasp selects either a caterpillar or a caterpillar egg and deposits a single, maybe a couple of eggs, in the host. The polyembryony is the fact that a single egg develops into anywhere from several hundred to several thousand larvae! Polyembryony isn’t confined to wasps. The pregnant female nine-banded armadillo invariably produces a set of four identical siblings of the same sex. Apparently, her uterus can only accommodate one implanted egg. However, that egg then splits into four genetically identical eggs. The polyembryonic wasp egg beats the armadillo egg by a factor of hundreds.
I have trouble coming to grips with what happens to that single wasp egg. The egg gives rise to these hundreds or thousands of genetically identical clones. When I think of clones, whatever the cloned creature, I think that the clones pretty much resemble each other. Not so with this wasp. There are two distinct types of larvae. One is the “soldier” larva, a snake-like little critter with mandibles that can chomp down on other larvae, as well as on the caterpillar. The other, “normal” wasp larva is more like a fat blob that goes on to develop into a full-grown wasp capable of reproduction.
The soldier larva is sterile and its primary function is to defend the normal larvae. When the latter pupate and get ready to emerge into the world as full-fledged wasps, the soldiers die. Research groups led by Ian Hardy of the University of Nottingham and by Mike Hardy of the University of Georgia have looked closely into the sibling interactions of the soldiers with other larvae. They’ve shown that the difference between the soldier and the normal larvae is that the normal larvae inherit a germ cell while the soldiers do not. Without the germ cell they can’t reproduce.
These researchers have studied the dining habits of the soldiers by labeling certain larvae with fluorescent tracers that light up the stomachs of the soldiers when they become items on the soldiers’ menus. The workers have also done experiments in which they introduce alien larvae and larvae of full brothers and sisters but are not genetically identical into a caterpillar that already contains a brood of developing larvae.
When the soldiers hatch, they launch into murdering other larvae. The investigators have found that, as any good sibling would do, they tend not to eat their closest relatives but concentrate on the aliens and the brothers or sisters that aren’t as closely related. How do they recognize their relatives? There’s something in the membrane that surrounds each larva that clues the marauding soldier about the kinship of his target. The researchers have performed microsurgery to remove membranes and transplant membranes and have demonstrated that it’s the membrane, not the larva, that determines whether the soldier eats the critter.
The soldier’s altruistic behavior in eating only his more distant siblings allows the normal larva who are his closest relatives to emerge as wasps and carry on the lineage. A surprising finding was that even when the food supply in the caterpillar was running short the soldiers still avoided eating their closest relatives. This reluctance to eat one’s close sibling, even in the face of starvation, remains a puzzle.
I’d like to thank my brother, and his wife, not only for a most pleasant visit and but also for providing material for a segue into the wasp sibling story. And, lest I leave the wrong impression, there is no puzzle should my brother and I be together and faced with starvation – I would not even consider eating him!
Allen F. Bortrum
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